Small subunit of the Chlamydomonas reinhardtii mitoribosome
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7pkq
Q-score: 0.231
Waltz F, Salinas-Giege T, Englmeier R, Meichel H, Soufari H, Kuhn L, Pfeffer S, Foerster F, Engel BD, Giege P, Drouard L, Hashem Y
To Be Published
- Bs16m (10 kDa, Protein from Chlamydomonas reinhardtii)
- Unk2 (12 kDa, Protein from Chlamydomonas smithii)
- Mitochondrial ribosomal protein s6 (13 kDa, Protein from Chlamydomonas reinhardtii)
- Bs1m (26 kDa, Protein from Chlamydomonas reinhardtii)
- Mitochondrial ribosomal protein s13 (13 kDa, Protein from Chlamydomonas reinhardtii)
- Ms105 (28 kDa, Protein from Chlamydomonas reinhardtii)
- Ms34 (14 kDa, Protein from Chlamydomonas reinhardtii)
- Unk4 (6 kDa, Protein from Chlamydomonas smithii)
- S5 drbm domain-containing protein (38 kDa, Protein from Chlamydomonas reinhardtii)
- Us12m (14 kDa, Protein from Chlamydomonas reinhardtii)
- Ms26 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Unk1 (2 kDa, Protein from Chlamydomonas smithii)
- Ms45 (102 kDa, Protein from Chlamydomonas reinhardtii)
- Ribosomal_s3_c domain-containing protein (50 kDa, Protein from Chlamydomonas reinhardtii)
- 30s ribosomal protein s9, chloroplastic (45 kDa, Protein from Chlamydomonas reinhardtii)
- Unk6 (48 kDa, Protein from Chlamydomonas smithii)
- S4 rrna (133 kDa, RNA from Chlamydomonas smithii)
- Plastid-specific ribosomal protein 4 (14 kDa, Protein from Chlamydomonas reinhardtii)
- Us8m (44 kDa, Protein from Chlamydomonas reinhardtii)
- S1 rrna (32 kDa, RNA from Chlamydomonas reinhardtii)
- Ms31/46 (15 kDa, Protein from Chlamydomonas smithii)
- Ms107 (43 kDa, Protein from Chlamydomonas reinhardtii)
- Us2m (53 kDa, Protein from Chlamydomonas reinhardtii)
- Us15m (34 kDa, Protein from Chlamydomonas reinhardtii)
- Us7m,ribosomal_s7 domain-containing protein,ribosomal_s7 domain-containing protein,ribosomal_s7 domain-containing protein (32 kDa, Protein from Chlamydomonas reinhardtii)
- Ms45-insert (36 kDa, Protein from Chlamydomonas smithii)
- S2 rrna (68 kDa, RNA from Chlamydomonas reinhardtii)
- Us17m (22 kDa, Protein from Chlamydomonas reinhardtii)
- Ribosomal_s10 domain-containing protein (61 kDa, Protein from Chlamydomonas reinhardtii)
- Us11m (23 kDa, Protein from Chlamydomonas reinhardtii)
- Ms35 (88 kDa, Protein from Chlamydomonas reinhardtii)
- Ms33 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Ms23 (20 kDa, Protein from Chlamydomonas reinhardtii)
- Ms106 (51 kDa, Protein from Chlamydomonas reinhardtii)
- Us4m (43 kDa, Protein from Chlamydomonas reinhardtii)
- Small subunit of the chlamydomonas reinhardtii mitochondrial ribosome (Complex from Chlamydomonas reinhardtii)
- Ms29 (49 kDa, Protein from Chlamydomonas reinhardtii)
- Bs18m (36 kDa, Protein from Chlamydomonas reinhardtii)
- Us19m (12 kDa, Protein from Chlamydomonas reinhardtii)
- Mitochondrial ribosomal protein s14 (13 kDa, Protein from Chlamydomonas reinhardtii)
- S3 rrna (126 kDa, RNA from Chlamydomonas reinhardtii)
- Unk3 (4 kDa, Protein from Chlamydomonas smithii)
- Unk5 (9 kDa, Protein from Chlamydomonas smithii)
Subtomogram average of the T. kivui 70S ribosome in situ
Dietrich HM, Righetto RD, Kumar A, Wietrzynski W, Trischler R, Schuller SK, Wagner J, Schwarz FM, Engel BD, Mueller V, Schuller JM
Nature (2022) 607 pp. 823-830 [ Pubmed: 35859174 DOI: doi:10.1038/s41586-022-04971-z ]
In situ cryo-electron tomography of the C. reinhardtii ciliary transition zone
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: 10.1126/science.abm6704 Pubmed: 35901159 ]
Image sets:
- CTF-corrected bin4 tomograms
- CTF-corrected bin2 tomograms
- Subtomograms at bin2
- Deconvoluted bin4 tomograms for visualization
- Unaligned tilt series raw frames
- Aligned tilt series stacks
In situ subtomogram average of the C. reinhardtii stellate at the ciliary transition zone
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]
In situ subtomogram average of the C. reinhardtii Y-link at the ciliary transition zone
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]
In situ subtomogram average of the C. reinhardtii MTD sleeve at the ciliary transition zone
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]
In situ subtomogram average of the C. reinhardtii MTD at the ciliary transition zone
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]
In situ subtomogram average of C. reinhardtii IFT-B at the ciliary transition zone
van den Hoek H, Klena N, Jordan MA, Alvarez Viar G, Righetto RD, Schaffer M, Erdmann PS, Wan W, Geimer S, Plitzko JM, Baumeister W, Pigino G, Hamel V, Guichard P, Engel BD
Science (2022) 377 pp. 543-548 [ DOI: doi:10.1126/science.abm6704 Pubmed: 35901159 ]